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Estimation of Haplotype Frequencies from Pooled Targeted Sequencing in Maize Landraces

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Raphaël, Minguella | Canaguier, Aurélie | Madur, Delphine | Hinsinger, Damien | Nicolas, Stephane | Faivre Rampant, Patricia

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International audience. Maizelandraces harbor a wider diversity than inbred lines and hybrids that canbe harnessed for plant breeding Although this diversity has been largely studiedin the past, much more remains to be done However, analyzing it requires togenotype many individuals per populations A solution to reduce the cost of thestudy is to genotype pools several plants of a given accession are mixed, thenthe DNA extraction is done in pool In addition, using short haplotypes usuallyimprove population structure inference but requires sequencing For thispurpose, we hijacked a targeted Genotyping By Sequencing tGBS technologyto sequence haplotypes from targeted genomics regions chosen among themany QTLs being known in maize

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