Syracuse

TRAP2 model for studying activated structures during Paradoxical (REM) Sleep

Archive ouverte

Malcey, Justin | Maciel, Renato | Chancel, Amarine | Cabrera, Sébastien | Fort, Patrice | Pierre-Hervé, Luppi

Edité par CCSD -

International audience. As postulated by Michel Jouvet as a paradoxal state, REM sleep exhibits EEG activities in cortical structures similar to those observed during wakefulness (W). However, for over a decade now, our results shows that only a few limbic cortical structures are activated during REM sleep in contrast to wakefulness. It confirms our hypotheses that REM sleep exhibit a pattern of neuronal activity state-related. Here, using TRAP2-red mice we propose to identify neurons activated during REM sleep. With this novel approach regarding sleep, we propose to broaden the avenue in the study of the REM sleep function(s). Aims : Taking advantage of the genetic engineering applied to animal models we propose in this study to identify neuronal populations activated during wakefulness and REM sleep using the TRAP2 mice. This knock-in strain allow us to get different time points in the same animal, and to obtain two fluorescent markers of neuronal activity: tdtomato and cFos. Corroborating our last findings, we identified a few structures exhibiting high cFos activity during REM sleep compared to wakefulness.

• Description
• Sujet/Public
• Outil
• Outil d'anticipation
• Mémoire et thèse
• Gestion