VASH1–SVBP and VASH2–SVBP generate different detyrosination profiles on microtubules

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Ramirez-Rios, Sacnicte | Choi, Sung, Ryul | Sanyal, Chadni | Blum, Thorsten, B | Bosc, Christophe | Krichen, Fatma | Denarier, Eric | Soleilhac, Jean-Marc | Blot, Béatrice | Janke, Carsten | Stoppin-Mellet, Virginie | Magiera, Maria, M | Arnal, Isabelle | Steinmetz, Michel, O | Moutin, Marie-Jo

Edité par CCSD ; Rockefeller University Press -

International audience. The detyrosination/tyrosination cycle of α-tubulin is critical for proper cell functioning. VASH1-SVBP and VASH2-SVBP are ubiquitous enzymes involved in microtubule detyrosination, whose mode of action is little known. Here, we show in reconstituted systems and cells that VASH1-SVBP and VASH2-SVBP drive the global and local detyrosination of microtubules, respectively. We solved the cryo-electron microscopy structure of VASH2-SVBP bound to microtubules, revealing a different microtubule-binding configuration of its central catalytic region compared to VASH1-SVBP. We show that the divergent mode of detyrosination between the two enzymes is correlated with the microtubule-binding properties of their disordered N-and C-terminal regions. Specifically, the N-terminal region is responsible for a significantly longer residence time of VASH2-SVBP on microtubules compared to VASH1-SVBP. We suggest that this VASH region is critical for microtubule detachment and diffusion of VASH-SVBP enzymes on lattices. Our results suggest a mechanism by which VASH1-SVBP and VASH2-SVBP could generate distinct microtubule subpopulations and confined areas of detyrosinated lattices to drive various microtubule-based cellular functions. .

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