TOXICOLOGICAL IMPACT OF SECONDARY ORGANIC AEROSOLS FORMED FROM THE REACTION OF LIMONENE WITH OZONE

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Jacob, Florence | Houzel, Nicolas | Alleman, Laurent | Perdrix, Esperanza | Anthérieu, Sébastien | Coeur, Cecile | Genevray, Paul | Garçon, Guillaume | Tomas, Alexandre | Lo-Guidice, Jean-Marc

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International audience. Secondary organic aerosols (SOAs) formed from the reaction of limonene with O3 were generated under weak and strong oxidation conditions in a flow reactor in order to evaluate their toxicity in relation to their physicochemical characteristics studied. SOAs were analysed with an SMPS (Scanning Mobility Particle Sizer) to measure the particle size distribution and by ESI-LC-QToF (ElectroSpray Ionization-Liquid Chromatography-Quadrupole-Time of Flight) to determine their chemical composition. Cell-free measurements of the intrinsic oxidative potential (OP) of the aerosols were performed using antioxidant depletions (DTT, AA) and a fluorescent probe (carboxyl-H2DCF-DA). In vitro tests, on the human bronchial epithelial BEAS-2B cells, were conducted to evaluate the oxidative and the inflammatory responses under various exposure conditions. The first results showed an intrinsic OP of the generated SOAs and the induction of antioxidant response-related parameters in exposed cells.

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