Afficher la couverture 'TFIIH plays an essential role in RNA polymerase I transcription | Iben, Sebastian' en grand format
/5

0 avis

TFIIH plays an essential role in RNA polymerase I transcription

Archive ouverte

Iben, Sebastian | Tschochner, Herbert | Bier, Mirko | Hoogstraten, Deborah | Hozák, Pavel | Egly, Jean-Marc | Grummt, Ingrid

Edité par CCSD ; Elsevier -

International audience. TFIIH is a multisubunit protein complex that plays an essential role in nucleotide excision repair and transcription of protein-coding genes. Here, we report that TFIIH is also required for ribosomal RNA synthesis in vivo and in vitro. In yeast, pre-rRNA synthesis is impaired in TFIIH ts strains. In a mouse, part of cellular TFIIH is localized within the nucleolus and is associated with subpopulations of both RNA polymerase I and the basal factor TIF-IB. Transcription systems lacking TFIIH are inactive and exogenous TFIIH restores transcriptional activity. TFIIH is required for productive but not abortive rDNA transcription, implying a postinitiation role in transcription. The results provide a molecular link between RNA polymerase I transcription and transcription-coupled repair of active ribosomal RNA genes.

Consulter en ligne

Suggestions

Du même auteur

CSB is a component of RNA pol I transcription

Archive ouverte | Bradsher, John | CCSD

Mutation in the CSB gene results in the human Cockayne's syndrome (CS). Here, we provide evidence that CSB is found not only in the nucleoplasm but also in the nucleolus within a complex (CSB IP/150) that contains RNA pol I, TFIIH...

Association of Yeast RNA Polymerase I with a Nucleolar Substructure Active in Rrna Synthesis and Processing

Archive ouverte | Fath, Stephan | CCSD

A novel ribonucleoprotein complex enriched in nucleolar proteins was purified from yeast extracts and constituents were identified by mass spectrometry. When isolated from rapidly growing cells, the assembly contained ribonucleic ...

DNA damage stabilizes interaction of CSB with the transcription elongation machinery

Archive ouverte | van den Boom, Vincent | CCSD

The Cockayne syndrome B (CSB) protein is essential for transcription-coupled DNA repair (TCR), which is dependent on RNA polymerase II elongation. TCR is required to quickly remove the cytotoxic transcription-blocking DNA lesions....

Chargement des enrichissements...