Draft genomes and descriptions of Urmitella timonensis gen. nov., sp. nov. and Marasmitruncus massiliensis gen. nov., sp. nov., isolated from severely malnourished African children using culturomics

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Bellali, Sara | Haddad, Gabriel | Pham, Thi-Phuong-Thao | Iwaza, Rim | Ibrahim, Ahmad | Armstrong, Nicholas | Fadlane, Amael | Couderc, Carine | Diallo, Aldiouma | Sokhna, Cheikh | Million, Matthieu | Raoult, Didier | Tidjani Alou, Maryam

Edité par CCSD ; Springer Verlag -

International audience. Two strains, designated as Marseille-P2918 T and Marseille-P3646 T , were isolated from a 14-week-old Senegalese girl using culturomics: Urmitella timonensis strain Marseille-P2918 T (= CSUR P2918, = DSM 103634) and Marasmitruncus massiliensis strain Marseille-P3646 T (= CSUR P3646, = CCUG72353). Both strains were rod-shaped, anaerobic, spore forming motile bacteria. The 16S rRNA gene sequences of strains Marseille-P2918 T (LT598554) and Marseille-P3646 T (LT725660) shared 93.25% and 94.34% identity with Tissierella praeacuta ATCC 25539 T and Anaerotruncus colihominis CIP 107754 T , their respective phylogenetically closest species with standing in nomenclature. Therefore, strain Marseille-P2918 T is classified within the family Tissierellaceae and order Tissierellales whereas strain Marseille-P3646 T is classified within the family Oscillospiraceae and order Eubacteriales . The genome of strain Marseille-P2918 T had a size of 2.13 Mb with a GC content of 50.52% and includes six scaffolds and six contigs, and that of strain Marseille-P3646 T was 3.76 Mbp long consisting of five contigs with a 50.04% GC content. The genomes of both strains presented a high percentage of genes encoding enzymes involved in genetic information and processing, suggesting a high growth rate and adaptability. These new taxa are extensively described and characterised in this paper, using the concept of taxono-genomic description.

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