Feedback on tGBS with Allegro : Does it hit the target ?

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Berard, Aurélie | Canaguier, Aurélie | Guilbaud, Romane | Le Clainche, Isabelle | Le Paslier, Marie-Christine | Loire, Benjamin | Hinsinger, Damien | Faivre Rampant, Patricia

Edité par CCSD -

International audience. SNP genotyping is widely used at many scale, both for research and breeding for diversity and genetic association studies, building genetic maps, confirm varieties identification, detect QTL and genomic selection. However, species diversity in term of genomic features (such as genome size, ploidy, heterozygosity) challenges the emergence of new and useful approaches. Chip arrays are considered as a "gold standard" but show limits in flexibility and associated costs. In addition, they return information on target SNP only, masking potential useful diversity around the target. We recently implemented a targeted Genotyping-By-Sequencing (tGBS) approach, namely the Allegro Targeted Genotyping v2 (TECAN).

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