Class III peroxidases PRX01, PRX44, and PRX73 potentially target extensins during root hair growth in Arabidopsis thaliana

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Marzol, Eliana | Borassi, Cecilia | Sardoy, Mariana, Carignani | Ranocha, Philippe | Aptekman, Ariel, A | Bringas, Mauro | Pennington, Janice | Paez-Valencia, Julio | Pacheco, Javier, Martínez | Rodríguez-Garcia, Diana, R | Rondón Guerrero, Yossmayer del Carmen | Peralta, Juan, Manuel | Fleming, Margaret | Mishler-Elmore, John, W | Mangano, Silvina | Blanco-Herrera, Francisca | Bedinger, Patricia | Dunand, Christophe | Capece, Luciana | Nadra, Alejandro, D | Held, Michael | Otegui, Marisa, S | Estevez, José, M

Edité par CCSD ; MDPI -

International audience. Root hair cells are important sensors of soil conditions. They grow towards and absorb water-soluble nutrients. This fast and oscillatory growth is mediated by continuous remodeling of the cell wall. Root hair cell walls contain polysaccharides and hydroxyproline-rich glycoproteins, including extensins (EXTs). Class-III peroxidases (PRXs) are secreted into the apoplastic space and are thought to trigger either cell wall loosening or polymerization of cell wall components, such as Tyr-mediated assembly of EXT networks (EXT-PRXs). The precise role of these EXT-PRXs is unknown. Using genetic, biochemical, and modeling approaches, we identified and characterized three root-hair-specific putative EXT-PRXs, PRX01, PRX44, and PRX73. prx01,44,73 triple mutation and PRX44 and PRX73 overexpression had opposite effects on root hair growth, peroxidase activity, and ROS production, with a clear impact on cell wall thickness. We use an EXT fluorescent reporter with contrasting levels of cell wall insolubilization in prx01,44,73 and PRX44-overexpressing background plants. In this study, we propose that PRX01, PRX44, and PRX73 control EXT-mediated cell wall properties during polar expansion of root hair cells

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