An interpretable and versatile machine learning approach for oocyte phenotyping

Archive ouverte

Letort, Gaelle | Eichmuller, Adrien | da Silva, Christelle | Nikalayevich, Elvira | Crozet, Flora | Salle, Jeremy | Minc, Nicolas | Labrune, Elsa | Wolf, Jean-Philippe | Terret, Marie-Emilie | Verlhac, Marie-Hélène

Edité par CCSD ; Company of Biologists -

International audience. Meiotic maturation is a crucial step of oocyte formation allowing its potential fertilization and embryo development. Elucidating this process is important both for fundamental research and assisted reproductive technology. Few computational tools based on non-invasive measurements are however available to characterize oocyte meiotic maturation. Here, we develop a computational framework to phenotype oocytes based on images acquired in transmitted light. We trained neural networks to segment the contour of oocytes and their zona pellucida using oocytes from diverse species. We defined a comprehensive set of morphological features to describe an oocyte. These steps are implemented in an open-source Fiji plugin. We present a feature based machine learning pipeline to recognize oocyte populations and determine their morphological differences. We first demonstrate its potential to screen oocyte from different strains and automatically identify their morphological characteristics. Its second application is to predict and characterize the maturation potential of oocytes. We identify the texture of the zona pellucida and the cytoplasmic particles size as features to assess mouse oocyte maturation potential and tested whether these features were applicable to human oocyte's developmental potential.

• Description
• Sujet/Public
• Outil
• Outil d'anticipation
• Mémoire et thèse
• Gestion