Fluorescent nanocarriers targeting VCAM-1 for early detection of senescent endothelial cells

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Belcastro, Eugenia | Rehman, Asad, Ur | Remila, Lamia | Park, Sin-Hee | Gong, Dal Seong | Anton, Nicolas | Auger, Cyril | Lefebvre, Olivier | Goetz, Jacky, G | Collot, Mayeul | Klymchenko, Andrey, S | Vandamme, Thierry, F | Schini-Kerth, Valérie, B

Edité par CCSD ; Elsevier -

International audience. Endothelial senescence has been identified as an early event in the development of endothelial dysfunction, a hallmark of cardiovascular disease. This study developed theranostic nanocarriers (NC) decorated with VCAM-1 antibodies (NC-VCAM-1) in order to target cell surface VCAM-1, which is overexpressed in senescent endothelial cells (ECs) for diagnostic and therapeutic purposes. Incubation of Ang IIinduced premature senescent ECs or replicative senescent ECs with NC-VCAM-1 loaded with lipophilic fluorescent dyes showed higher fluorescence signals than healthy EC, which was dependent on the NC size and VCAM-1 antibodies concentration, and not observed following masking of VCAM-1. NC loaded with omega 3 polyunsaturated fatty acid (NC-EPA:DHA6:1) were more effective than native EPA:DHA 6:1 to prevent Ang II-induced VCAM-1 and p53 upregulation, and SA-β-galactosidase activity in coronary artery segments. These theranostic NC might be of interest to evaluate the extent and localization of endothelial senescence and to prevent pro-senescent endothelial responses.

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