Alignment of poplar linkage maps through SSR markers genetic mapping

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Jorge, Véronique, V. | Bert, Pierre-François, P.-F.

Edité par CCSD -

International audience. Several linkage maps have been produced for genus Populus. One of them, constructed at INRA Orléans have been based on 91 F1 progeny derived from an interspecific cross between two P. deltoides and P. trichocarpa clones, using double pseudo-testcross mapping strategy and different types of markers (AFLPs, RAPDs, RFLPs, SSRs, STSs, including genes with known function). RFLPs, SSRs and STSs mapped allowed only very low alignment with the poplar maps published. Additionally, these maps were not saturated. Interspecific F1 progeny used has been increased to 342 F1 genotypes to improved recombination frequency estimations. As a part of the POPYOMICS European project and in order to improved the map alignment and saturation, we used publicly available SSR primer sequence corresponding to markers already mapped. Fluorescent genotyping using M13 tailed primer technique and ABI PRISM sequencer has been used. Two to 5 markers by linkage groups were chosen to cover the 19 linkage groups defined previously corresponding to a total of more than 100 SSR primers. Additionally, 16 SSR markers were developed mining poplar EST databases (so-called EST-SSR markers). This new linkage map will be used in comparative genetics and QTL mapping and EST based alignment with poplar genome sequence.

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