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TREC-IN: gene knock-in genetic tool for genomes cloned in yeast.

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Chandran, Suchismita | Noskov, Vladimir N | Segall-Shapiro, Thomas H | Ma, Li | Whiteis, Caitlin | Lartigue, Carole | Jores, Joerg | Vashee, Sanjay | Chuang, Ray-Yuan

Edité par CCSD ; BioMed Central -

International audience. Although we have demonstrated the feasibility of TREC-IN in gene complementation and genome minimization studies in Mmc, the applicability of TREC-IN ranges widely. This method proves to be a valuable genetic tool that can be extended for genomic engineering in other genetically intractable organisms, where it may be implemented in elucidating specific metabolic pathways and in rationale vaccine design.

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