Nidogen-1 Contributes to the Interaction Network Involved in Pro-B Cell Retention in the Peri-sinusoidal Hematopoietic Stem Cell Niche

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Balzano, Marielle | de Grandis, Maria | Vu Manh, Thien-Phong | Chasson, Lionel | Bardin, Florence | Farina, Anne | Sergé, Arnauld | Bidaut, Ghislain | Charbord, Pierre | Hérault, Léonard | Bailly, Anne-Laure | Cartier-Michaud, Amandine | Boned, Annie | Dalod, Marc | Duprez, Estelle | Genever, Paul | Coles, Mark, C | Bajenoff, Marc | Xerri, Luc | Aurrand-Lions, Michel | Schiff, Claudine | Mancini, Stéphane J.C.

Edité par CCSD ; Elsevier Inc -

International audience. In the bone marrow, CXCL12 and IL-7 are essential for B cell differentiation, whereas hematopoietic stem cell (HSC) maintenance requires SCF and CXCL12. Peri-sinusoidal stromal (PSS) cells are the main source of IL-7, but their characterization as a pro-B cell niche remains limited. Here, we characterize pro-B cell supporting stromal cells and decipher the interaction network allowing pro-B cell retention. Preferential contacts are found between pro-B cells and PSS cells, which homogeneously express HSC and B cell niche genes. Furthermore, pro-B cells are frequently located in the vicinity of HSCs in the same niche. Using an interactome bioinformatics pipeline, we identify Nidogen-1 as essential for pro-B cell retention in the peri-sinusoidal niche as confirmed in Nidogen-1-/- mice. Finally, human pro-B cells and hematopoietic progenitors are observed close to similar IL-7+ stromal cells. Thus, a multispecific niche exists in mouse and human supporting both early progenitors and committed hematopoietic lineages.

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