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Functional Characterization of the Arabidopsis Abscisic Acid Transporters NPF4.5 and NPF4.6 in Xenopus Oocytes

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Léran, Sophie | Noguero, Mélanie | Corratgé-Faillie, Claire | Boursiac, Yann | Brachet, Chantal | Lacombe, Benoît

Edité par CCSD ; Frontiers -

Dynamic reprogramming of gene regulatory networks (GRNs) enables organisms to rapidlyrespond to environmental perturbation. However, the underlying transient interactionsbetween transcription factors (TFs) and genome-wide targets typically elude biochemicaldetection. Here, we capture both stable and transient TF-target interactions genome-widewithin minutes after controlled TF nuclear import using time-series chromatin immunoprecipitation(ChIP-seq) and/or DNA adenine methyltransferase identification (DamID-seq).The transient TF-target interactions captured uncover the early mode-of-action of NIN-LIKEPROTEIN 7 (NLP7), a master regulator of the nitrogen signaling pathway in plants. Thesetransient NLP7 targets captured in root cells using temporal TF perturbation account for 50%of NLP7-regulated genes not detectably bound by NLP7 in planta. Rapid and transient NLP7binding activates early nitrogen response TFs, which we validate to amplify the NLP7-initiatedtranscriptional cascade. Our approaches to capture transient TF-target interactions genomewidecan be applied to validate dynamic GRN models for any pathway or organism of interest.

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