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Purification of Tubulin with Controlled Posttranslational Modifications and Isotypes from Limited Sources by Polymerization-Depolymerization Cycles

Archive ouverte | Bodakuntla, Satish | CCSD

International audience. One important aspect of studies of the microtubule cytoskeleton is the investigation of microtubule behavior in in vitro reconstitution experiments. They allow the analysis of the intrinsic p...

Tubulin polyglutamylation is a general traffic-control mechanism in hippocampal neurons

Archive ouverte | Bodakuntla, Satish | CCSD

International audience. Neurons are highly complex cells that heavily rely on intracellular transport to distribute a range of functionally essential cargoes within the cell. Post-translational modifications of tubu...

Purification of tubulin with controlled post-translational modifications by polymerization–depolymerization cycles

Archive ouverte | Souphron, Judith | CCSD

International audience. In vitro reconstitutions of microtubule assemblies have provided essential mechanistic insights into the molecular bases of microtubule dynamics and their interactions with associated protein...

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