Electrotransfer of CpG free plasmids enhances gene expression in skin

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Chabot, S. | Bellard, E. | Reynes, J.P. | Tiraby, G. | Teissié, J. | Golzio, Muriel

Edité par CCSD ; Elsevier -

International audience. Skin is a very suitable target for gene therapy and DNA vaccination due to its accessibility, its surface, its ability to produce transgenes. Gene electrotransfer (GET) to the skin is under development for clinical applications for DNA vaccine or local treatment such as wound healing. Local treatments are effective if expression of the plasmid affects only the local environment (skin) by inducing an efficient concentration over a prolonged period. In this study, we evaluate the control of expression in the skin of a plasmid coding a fluorescent protein by its CpG (cytosinephosphate-guanine motif) content. Two fluorescent reporter genes are evaluated: dTomato and GFP. Expression is followed on a long term by in vivo fluorescence imaging. Our results show that GET mediated expression in the skin can be controlled by the CpG content of the plasmid. Expression on the long term (> 120 days) can be obtained at a high level with CpG-free constructs associated to a proper design of the electrodes where a field distribution mediating the gene electrotransfer is present deep in the skin.

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