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Single molecule kinetics uncover roles for E. coli RecQ DNA helicase domains and interaction with SSB

Archive ouverte | Bagchi, Debjani | CCSD

International audience. Most RecQ DNA helicases share a conserved domain arrangement that mediates their activities in genomic stability. This arrangement comprises a helicase motor domain, a RecQ C-terminal (RecQ-C...

A conserved structural element in the RNA helicase UPF1 regulates its catalytic activity in an isoform-specific manner

Archive ouverte | Gowravaram, Manjeera | CCSD

International audience. The RNA helicase UPF1 is a key component of the nonsense mediated mRNA decay (NMD) pathway. Previous X-ray crystal structures of UPF1 elucidated the molecular mechanisms of its catalytic acti...

Displacement and dissociation of oligonucleotides during DNA hairpin closure under strain

Archive ouverte | Ding, Fangyuan | CCSD

International audience. The hybridization kinetic of an oligonucleotide to its template is a fundamental step in many biological processes such as replication arrest, CRISPR recognition, DNA sequencing, DNA origami,...

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