Proteome-wide drug and metabolite interaction mapping by thermal-stability profiling

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Proteome-wide drug and metabolite interaction mapping by thermal-stability profiling

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Edité par CCSD ; Nature Publishing Group -

International audience. Thermal stabilization of proteins after ligand binding provides an efficient means to assess the binding of small molecules to proteins. We show here that in combination with quantitative mass spectrometry, the approach allows for the systematic survey of protein engagement by cellular metabolites and drugs. We profiled the targets of the drugs methotrexate and (S)-crizotinib and the metabolite 2'3'-cGAMP in intact cells and identified the 2'3'-cGAMP cognate transmembrane receptor STING, involved in immune signaling.

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