Liquid and gas residence time distribution in a two-stage bioreactor with cell recycle

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Ben Gaida, Lamia | Andre, Christophe | Bideaux, Carine | Alfenore, Sandrine | Cameleyre, Xavier | Molina-Jouve, Carole | Fillaudeau, Luc

Edité par CCSD -

Abstract: Hydrodynamic behaviour of a two-stage bioreactor with cell recycle (TSCB) was investigated. Residence time distribution (RTD) and Internal Age Distribution (AID) of gas and liquid phases were performed with accurate tracing methods and under specific operating conditions and process modes (batch and continuous modes with and without recirculation between first and second stages). From experiments, RTD and analytical solution were formulated, and systemic analysis carried out. Results demonstrated that the magnitude of mean residence time and variance were strongly different between gas (»1min) and liquid (»100min) phases. RTD of gas phase showed that both reactors exhibited independent behaviour, and RTD could be described by two single and simple models (plug flow in series with j CSTR) with constant parameters (a, j) whatever investigated operating conditions (VVM, Re and Remixing). For liquid phase, experiments will enable to identify mixing time (batch mode) as well as the mean residence time and variance in continuous modes. RTD for permeate and purge flow and IAD in the first and second reactors were obtained and formulated. Under selective assumptions, a systemic analysis of liquid RTD lead to propose a reactor model in close relation with TSCB structure. In a first step, model parameters will be identified under continuous mode without recirculation and then, validated with recycle between two stages.
Abstract: Hydrodynamic behaviour of a two-stage bioreactor with cell recycle (TSCB) was investigated. Residence time distribution (RTD) and Internal Age Distribution (AID) of gas and liquid phases were performed with accurate tracing methods and under specific operating conditions and process modes (batch and continuous modes with and without recirculation between first and second stages). From experiments, RTD and analytical solution were formulated, and systemic analysis carried out. Results demonstrated that the magnitude of mean residence time and variance were strongly different between gas (»1min) and liquid (»100min) phases. RTD of gas phase showed that both reactors exhibited independent behaviour, and RTD could be described by two single and simple models (plug flow in series with j CSTR) with constant parameters (a, j) whatever investigated operating conditions (VVM, Re and Remixing). For liquid phase, experiments will enable to identify mixing time (batch mode) as well as the mean residence time and variance in continuous modes. RTD for permeate and purge flow and IAD in the first and second reactors were obtained and formulated. Under selective assumptions, a systemic analysis of liquid RTD lead to propose a reactor model in close relation with TSCB structure. In a first step, model parameters will be identified under continuous mode without recirculation and then, validated with recycle between two stages.. Liquid and gas residence time distribution in a two-stage bioreactor with cell recycle. Tracer5, Tracers and Tracing methods

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