FISH-in-CHIPS: A Microfluidic Platform for Molecular Typing of Cancer Cells

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Perez-Toralla, Karla | Mottet, Guillaume | Tulukcuoglu-Guneri, Ezgi | Champ, Jérôme | Bidard, François-Clément | Pierga, Jean-Yves | Klijanienko, Jerzy | Draskovic, Irena | Malaquin, Laurent | Viovy, Jean-Louis | Descroix, Stéphanie | Venzac, B. | Diakité, L. | Herthnek, D. | Cissé, I. | Bockelmann, U.

Edité par CCSD ; Royal Society of Chemistry -

International audience. Microfluidics offer powerful tools for the control, manipulation, and analysis of cells, in particular for theassessment of cell malignancy or the study of cell subpopulations. However, implementing complex biologicalprotocols on chip remains a challenge. Sample preparation is often performed off chip using multiplemanually performed steps, and protocols usually include different dehydration and drying steps thatare not always compatible with a microfluidic format.Here, we report the implementation of a Fluorescence in situ Hybridization (FISH) protocol forthe molecular typing of cancer cells in a simple and low-cost device. The geometry of the chip allowsintegrating the sample preparation steps to efficiently assess the genomic content of individual cellsusing a minute amount of sample. The FISH protocol can be fully automated, thus enabling its use inroutine clinical practice.

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