PSF engineering in multifocus microscopy for increased depth volumetric imaging

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Hajj, Bassam | El Beheiry, Mohamed | Dahan, Maxime

Edité par CCSD ; Optical Society of America - OSA Publishing -

International audience. Imaging and localizing single molecules with high accuracy in a 3D volume is a challenging task. Here we combine multifocal microscopy, a recently developed volumetric imaging technique, with point spread function engineering to achieve an increased depth for single molecule imaging. Applications in 3D single molecule localization-based super-resolution imaging is shown over an axial depth of 4 µm as well as for the tracking of diffusing beads in a fluid environment over 8 µm.

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