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Which phosphatases may contribute to P use efficiency for N2 fixation in the rhizobial symbiosis with legumes ?
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Edité par CCSD ; Universitad de Sevilla -
In order to assess the contribution of phosphatases activity to P use efficiency for N2 fixation in legume nodules, an in situ RT-PCR methodology was used to localize and quantify the transcripts of candidate phosphatases genes in nodules of common bean in hydro-aeroponic culture under deficient vs sufficient P supply. The transcript localization of phytase, phosphoenolpyruvate-, fructose 1,6 bisphosphate- and trehalose 6-phosphate- phosphatases was found to be tissue specific and to differ among Pase genes, P treatment and legume genotype. It is concluded that these phosphatases contribute differently to the use of organic P for N2 fixation and the increase in nodule permeability to O2 under P deficiency.
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