Comparison of four commercial real-time PCR assays for detection of Mycobacterium tuberculosis complex, associated or not to detection of anti-tuberculosis drug-resistance genes

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Berger, Sibel | Laurain, Catherine | Larcher, Christine | Lozniewski, Alain | Alauzet, Corentine

Edité par CCSD -

International audience. BACKGROUNDTuberculosis (TB) is one of the world’s most important infectious causes of morbidity and mortality.Emergence and large spread of drug-resistant TB cause major therapeutic problems worldwide.Early detection, with rapid and reliable diagnosis methods, is essential to reduce the death rate and interrupt transmission of TB, particularly drug-resistant TB.Various PCR-based assays are currently available and they enable the direct and fast detection of the Mycobacterium tuberculosis complex (MTBC) in clinical samples.Some of these kits also permit the detection of resistance to the most important anti-tuberculosis drugs.However, their sensitivity varies according to the technique or the type of clinical sample, particularly in the case of smear-negative specimens.OBJECTIVESComparison of diagnostic performances of four commercial PCR-based kits for the direct detection of MTBC in respiratory and non-respiratory samples.Evaluation of two of them for their ability to detect resistance genes to rifampicin (RMP) and/or isoniazid (INH).

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