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Mapping of the epitopes of poliovirus type 2 in complex with antibodies
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International audience. The inactivated polio vaccine (IPV) contains poliovirus (PV) samples that belong to serotypes 1, 2 and 3.All three serotypes contain the D-antigen, which induces protective antibodies. The antigenic structureof PVs consists of at least four different antigenic sites and the D-antigen content represents the combinedactivity of multiple epitopes (Ferguson et al., 1993; Minor, 1990; Minor et al., 1986). The potencyof IPV vaccines is determined by measuring the D-antigen content. Several ELISA methods have beendeveloped using polyclonal or monoclonal antibodies (Mabs) in order to quantify the D-antigen content.Characterization of the epitopes recognized by the different Mabs is crucial to map the entire virus surfaceand ensure the presence of epitopes able to induce neutralizing antibodies. Using a new approachthat we developed to study the interaction between monoclonal antibodies and poliovirus type 2, whichcombines cryo-electron microscopy, image analysis and X-ray crystallography along with identificationof exposed amino acids, we have mapped in 3D the epitope sites recognized by three specific Fabs at thesurface of poliovirus type 2 (PV2) and characterized precisely the antigenic sites for these Fabs.
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