Afficher la couverture 'Action spectroscopy of a protonated peptide in the ultraviolet range | Canon, Francis' en grand format
/5

0 avis

Action spectroscopy of a protonated peptide in the ultraviolet range

Archive ouverte

Canon, Francis | Milosavljević, Aleksandar R. | Nahon, Laurent | Giuliani, Alexandre

Edité par CCSD ; Royal Society of Chemistry -

Action spectroscopy of protonated substance P, a model undecapeptide, has been probed from 5.2 eV to 20 eV. For photon energy above the ionization threshold measured at 10.3 ± 0.1 eV, the radical dication is observed along with side chain losses and abundant formation of all kinds of sequence ions. Below the photoionization threshold, the photoproducts involve side chain cleavages and backbone cleavages into a-, b-/y-, and c-sequence ions. Different electronic excited states appear to produce different fragment ions. Norrish type I and II reactions are proposed to account for some photoproducts. This study bridges the gap between laser activation and electron-induced dissociation of peptides. Moreover, our results report for the first time a comprehensive picture of the photochemical fragmentation of a gas phase peptide in a wide photon energy range.

Consulter en ligne

Suggestions

Du même auteur

Application of VUV synchroton radiation to proteomic and analytical mass spectrometry

Archive ouverte | Giuliani, Alexandre | CCSD

Application of VUV synchroton radiation to proteomic and analytical mass spectrometry. 11. international conference on Synchroton radiation instrumentation (Sri 2012)

Photodissociation of protonated Leucine-Enkephalin peptide in the VUV range

Archive ouverte | Ranković, Milos Lj. | CCSD

We present results of VUV action spectroscopy of gas-phase protonated Leucin-Enkephalin peptide. The experiment has been performed by coupling a linear quadrupole ion trap with a synchrotron radiation VUV beamline.

K-shell excitation and ionization of a gas-phase protein: interplay between electronic structure and protein folding

Archive ouverte | Milosavljević, Aleksandar R. | CCSD

Understanding the correlation between proteins' tertiary and electronic structures is a great challenge, which could possibly lead to a more efficient prediction of protein functions in living organisms. Here, we report an experim...

Chargement des enrichissements...