The dynamics of oil bodies in A. thaliana seeds :A mathematical model of biogenesis and coalescence

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Trigui, Ghassen | Laroche, Béatrice | Miquel, Martine | Dubreucq, Bertrand | Trubuil, Alain

Edité par CCSD -

The subcellular organelles called oil bodies (OBs) arelipid-filled quasi-spherical droplets produced from the endoplasmicreticulum (ER) and then released into the cytoplasm during seeddevelopment. It is believed that an OB grows by coalescence withother OBs and that its stability depends on the composition ofoleosins, major proteins inserted in the hemi membrane that coversOBs. In this study, we measured the OB-volume distribution fromdifferent genotypes of A. thaliana after 7, 8, 9, 10 and 11 days ofseed development. In order to test the hypothesis of OBs dynamics,we developed a simple mathematical model using non-linear differentialequations inspired from the theory of coagulation. The modeldescribes the evolution of OB-volume distribution during the firststeps of seed development by taking into consideration the productionof OBs, the increase of triacylglycerol volume to be stored, and thegrowth by coalescence of OBs. Fitted parameters values show anincrease in the OB production and coalescence rates in A. thalianaoleosin mutants compared to wild type.

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