Protein tyrosine kinase 7 has a conserved role in Wnt/β-catenin canonical signalling.

Archive ouverte

Puppo, Francesca | Thomé, Virginie | Lhoumeau, Anne-Catherine | Cibois, Marie | Gangar, Akanksha | Lembo, Frédérique | Belotti, Edwige | Marchetto, Sylvie | Lécine, Patrick | Prébet, Thomas | Sebbagh, Michael | Shin, Won-Sik | Lee, Seung-Taek | Kodjabachian, Laurent | Borg, Jean-Paul

Edité par CCSD ; EMBO Press -

International audience. The receptor protein tyrosine kinase 7 (PTK7) was recently shown to participate in noncanonical Wnt/planar cell polarity signalling during mouse and frog embryonic development. In this study, we report that PTK7 interacts with β-catenin in a yeast two-hybrid assay and mammalian cells. PTK7-deficient cells exhibit weakened β-catenin/T-cell factor transcriptional activity on Wnt3a stimulation. Furthermore, Xenopus PTK7 is required for the formation of Spemann's organizer and for Siamois promoter activation, events that require β-catenin transcriptional activity. Using epistatic assays, we demonstrate that PTK7 functions upstream from glycogen synthase kinase 3. Taken together, our data reveal a new and conserved role for PTK7 in the Wnt canonical signalling pathway.

• Description
• Sujet/Public
• Outil
• Outil d'anticipation
• Mémoire et thèse
• Gestion