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Parainfluenza virus type 5 (PIV-5) morphology revealed by cryo-electron microscopy.

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Terrier, Olivier | Rolland, Jean-Paul | Rosa-Calatrava, Manuel | Lina, Bruno | Thomas, Daniel | Moules, Vincent

Edité par CCSD ; Elsevier -

International audience. The knowledge of parainfluenza type 5 (PIV-5) virion morphology is essentially based on the observation of negatively stained preparations in conventional transmission electron microscopy (CTEM). In this study, the ultrastructure of frozen-hydrated intact PIV-5 was examined by cryo-electron microscopy (cryo-EM). Cryo-EM revealed a majority of spherical virions (70%), with a lower pleiomorphy than originally observed in CTEM. Phospholipid bilayer thickness, spike length and glycoprotein spikes density were measured. About 2000 glycoprotein spikes were present in an average-sized spherical virion. Altogether, these data depict a more precise view of PIV-5 morphology.

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