Carotid chemodenervation approach to study oxygen sensing in brain stem catecholaminergic cells

Archive ouverte

Pascual, O. | Roux, J.C. | Soulage, C. | Morin-Surun, M.P. | Denavit-Saubie, M. | Pequignot, J.M.

Edité par CCSD ; Elsevier -

This article addresses physiological conditions of hypoxia known as moderate or tolerable hypoxia, which allows long-term exposure lasting for weeks. The exposure to tolerable hypoxia will enable us to define the morphofunctional mechanisms involved in the ventilatory acclimatization of intact or carotid chemodenervated animals. Moreover, exposure to moderate hypoxia induces the activation of brain stem catecholaminergic cells located in cardiorespiratory areas and+45 degree ruleor involved in the modulation of cardiorespiratory output. Such hypoxic conditions can be reproduced easily either in vivo by subjecting small rodents (rats, mice, guinea pigs) to a gas mixture in which the oxygen level has been lowered below 20.9% or in vitro by superfusing isolated brain stem or brain stem slices using an artificial cerebrospinal fluid saturated with a hypoxic gas mixture. In order to define oxygen sensing mechanisms in carotid chemodenervated animals, this article successively describes the in vivo and in vitro preparations used to test the effect of a moderate hypoxia on brain stem respiratory areas and then the methods used to evaluate the consequences of hypoxia on ventilation, catecholamine neurochemistry, and gene expression.

• Description
• Sujet/Public
• Outil
• Outil d'anticipation
• Mémoire et thèse
• Gestion